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mouse monoclonal anti plcβ2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal anti plcβ2
    Mouse Monoclonal Anti Plcβ2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+plc%CE%B22/PLC+%CE%B22+Antibody/pm36982874-387-64-68
    Average 93 stars, based on 101 article reviews
    mouse monoclonal anti plcβ2 - by Bioz Stars, 2026-10
    93/100 stars

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    Blocking Assay:

    Article Title: Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36
    Article Snippet: .. Then, samples were placed in a blocking buffer (1% bovine serum albumin, 0.1% Tween-20 in PBS) for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C: rabbit polyclonal anti-AdipoR1 (1:50; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-CD36 (1:50; Abcam, Boston, MA, USA), rabbit polyclonal anti-CD36 (1:50; Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal anti-GPR120 (1:50; Santa Cruz Biotechnology), mouse monoclonal anti-PLCβ2 (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-Gα-gust (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-TRPM5 (1:100; Alomone Labs). .. After washing three times with PBS, the coverslips were incubated with goat anti-rabbit Alexa Fluor 594 (1:500, Invitrogen) and/or goat anti-mouse AlexaFluor488 (1:500, Invitrogen) for 1.5 h at room temperature.

    Article Title: Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36.
    Article Snippet: Statistical analysis was performed using either a one-sample t-test, unpaired Student’s t-test, or a one-way ANOVA with Tukey test for post hoc multiple comparisons in GraphPad Prism 9 (Version 9.5.0 (730), GraphPad Software, Boston, MA, USA) as appropriate and described in each experiment above. .. Then, samples were placed in a blocking buffer (1% bovine serum albumin, 0.1% Tween-20 in PBS) for 1 h at room temperature and incubated with primary antibodies overnight at 4 ◦C: rabbit polyclonal anti-AdipoR1 (1:50; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-CD36 (1:50; Abcam, Boston, MA, USA), rabbit polyclonal anti-CD36 (1:50; Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal anti-GPR120 (1:50; Santa Cruz Biotechnology), mouse monoclonal anti-PLCβ2 (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-Gα-gust (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-TRPM5 (1:100; Alomone Labs). .. After washing three times with PBS, the coverslips were incubated with goat anti-rabbit Alexa Fluor 594 (1:500, Invitrogen) and/or goat anti-mouse AlexaFluor488 (1:500, Invitrogen) for 1.5 h at room temperature.

    Incubation:

    Article Title: Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36
    Article Snippet: .. Then, samples were placed in a blocking buffer (1% bovine serum albumin, 0.1% Tween-20 in PBS) for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C: rabbit polyclonal anti-AdipoR1 (1:50; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-CD36 (1:50; Abcam, Boston, MA, USA), rabbit polyclonal anti-CD36 (1:50; Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal anti-GPR120 (1:50; Santa Cruz Biotechnology), mouse monoclonal anti-PLCβ2 (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-Gα-gust (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-TRPM5 (1:100; Alomone Labs). .. After washing three times with PBS, the coverslips were incubated with goat anti-rabbit Alexa Fluor 594 (1:500, Invitrogen) and/or goat anti-mouse AlexaFluor488 (1:500, Invitrogen) for 1.5 h at room temperature.

    Article Title: Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36.
    Article Snippet: Statistical analysis was performed using either a one-sample t-test, unpaired Student’s t-test, or a one-way ANOVA with Tukey test for post hoc multiple comparisons in GraphPad Prism 9 (Version 9.5.0 (730), GraphPad Software, Boston, MA, USA) as appropriate and described in each experiment above. .. Then, samples were placed in a blocking buffer (1% bovine serum albumin, 0.1% Tween-20 in PBS) for 1 h at room temperature and incubated with primary antibodies overnight at 4 ◦C: rabbit polyclonal anti-AdipoR1 (1:50; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-CD36 (1:50; Abcam, Boston, MA, USA), rabbit polyclonal anti-CD36 (1:50; Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal anti-GPR120 (1:50; Santa Cruz Biotechnology), mouse monoclonal anti-PLCβ2 (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-Gα-gust (1:50; Santa Cruz Biotechnology), rabbit polyclonal anti-TRPM5 (1:100; Alomone Labs). .. After washing three times with PBS, the coverslips were incubated with goat anti-rabbit Alexa Fluor 594 (1:500, Invitrogen) and/or goat anti-mouse AlexaFluor488 (1:500, Invitrogen) for 1.5 h at room temperature.



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    93
    Santa Cruz Biotechnology mouse monoclonal anti plcβ2
    Mouse Monoclonal Anti Plcβ2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+plc%CE%B22/PLC+%CE%B22+Antibody/pm36982874-387-64-68
    Average 93 stars, based on 1 article reviews
    mouse monoclonal anti plcβ2 - by Bioz Stars, 2026-10
    93/100 stars
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